The Human VEGFR3 (FLT4) CLIA Kit is a cutting-edge assay designed to accurately measure levels of Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) in human samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and consistent results that are crucial for a wide array of research applications.VEGFR3, also known as FLT4, is a key receptor involved in vascular endothelial cell growth and plays a crucial role in angiogenesis.
Its dysregulation has been linked to various diseases, including cancer, cardiovascular diseases, and lymphatic disorders. Therefore, the Human VEGFR3 (FLT4) CLIA Kit serves as a valuable tool for studying these conditions and potentially developing targeted therapies.Overall, the Human VEGFR3 (FLT4) CLIA Kit is a must-have for researchers seeking to deepen their understanding of vascular biology and explore novel treatment strategies for diseases associated with aberrant angiogenesis.
Human VEGFR3/Flt4 (Vascular Endothelial Cell Growth Factor Receptor 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VEGFR3/Flt4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGFR3/Flt4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGFR3/Flt4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VEGFR3/Flt4. You can calculate the concentration of Human VEGFR3/Flt4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
85-98
101-118
Average (%)
98
90
109
1:4
Range (%)
100-114
85-97
92-107
Average (%)
107
91
99
1:8
Range (%)
91-106
102-119
96-110
Average (%)
99
109
101
1:16
Range (%)
83-96
98-115
102-118
Average (%)
90
106
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
104
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
98-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.42
245.31
675.09
97.36
257.1
737.44
Standard deviation
12.74
17.86
68.39
12.47
20.62
76.69
C V (%)
12.69
7.28
10.13
12.81
8.02
10.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VEGFR3/Flt4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGFR3/Flt4 in samples. No significant cross-reactivity or interference between Human VEGFR3/Flt4 and analogues was observed.
