Human VLDL R/Very low-density lipoprotein receptor ELISA Kit
The Human VLDLR (Very Low Density Lipoprotein Receptor) ELISA Kit is a highly reliable and accurate tool for the measurement of VLDLR levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing precise and consistent results for a variety of research applications. The VLDLR protein serves a critical function in lipid metabolism, specifically in the clearance of circulating very low-density lipoproteins (VLDL) from the bloodstream. Dysregulation of VLDLR has been implicated in various metabolic disorders, including atherosclerosis, hyperlipidemia, and insulin resistance.
Therefore, the Human VLDLR ELISA Kit is an invaluable resource for studying these conditions and exploring potential therapeutic interventions. With its advanced technology and proven performance, the Human VLDLR ELISA Kit is an essential tool for researchers and scientists seeking to gain insights into VLDLR biology and its role in metabolic disorders. Trust in this kit to deliver accurate and dependable results, contributing to advancements in biomedical research and drug development.
Product Name:
Human VLDL R/Very low-density lipoprotein receptor ELISA Kit
SKU:
HUFI00869
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-101%
92-99%
85-104%
EDTA Plasma (n = 5)
92-99%
90-101%
83-100%
Heparin Plasma (n = 5)
83-99%
83-99%
84-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
89-103
94
EDTA Plasma (n = 5)
85-100
95
Heparin Plasma (n = 5)
90-104
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
