The Human VWF (Von Willebrand Factor) CLIA Kit is specifically designed for the quantitative measurement of Von Willebrand Factor levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Von Willebrand Factor is a key protein involved in blood clotting and plays a crucial role in conditions such as Von Willebrand disease and other bleeding disorders.
Elevated levels of VWF have also been linked to cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing new treatment strategies.Overall, the Human VWF CLIA Kit provides a reliable tool for researchers to examine VWF levels and better understand its role in various physiological and pathological processes.
Product Name:
Human VWF (Von Willebrand Factor) CLIA Kit
SKU:
HUES01145
Target:
Human VWF (Von Willebrand Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VWF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VWF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VWF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VWF. You can calculate the concentration of Human VWF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
95-106
90-101
Average (%)
103
101
96
1:4
Range (%)
103-117
94-108
93-107
Average (%)
110
100
101
1:8
Range (%)
101-114
97-112
92-104
Average (%)
108
105
99
1:16
Range (%)
101-117
92-105
83-96
Average (%)
107
97
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
95-109
103
Cell culture media (n=5)
85-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.02
2.47
10.0
0.92
2.44
9.95
Standard deviation
0.1
0.18
0.81
0.11
0.23
0.79
C V (%)
9.8
7.29
8.1
11.96
9.43
7.94
Sample type &Sample volume:
Plasma; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VWF concentrations in Plasma.
Specificity:
This kit recognizes Human VWF in samples. No significant cross-reactivity or interference between Human VWF and analogues was observed.
