The IL2RA Antibody (CAB2984) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.IL2RA plays a crucial role in the immune system, particularly in the activation and proliferation of T cells. Dysregulation of IL2RA has been implicated in various autoimmune diseases and inflammatory conditions, making it a key target for research in immunology and cancer biology.
This antibody is validated for use in WB, IHC-P, FC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IL2RA Antibody
SKU:
CAB2984
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p55, CD25, IL2R, IMD41, TCGFR, IDDM10
Positive Sample:
Rat spleen, Rat lung
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
31kDa
Observed MW:
60kDa
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk.
Purification Method
Affinity purification
Gene ID
3559
RRID
AB_2764795
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide,pH7.3.
Western blot analysis of various lysates using CD25 Rabbit pAb (CAB2984) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using CD25 Rabbit pAb (CAB2984) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human gastric cancer tissue using CD25 Rabbit pAb (CAB2984) at a dilution of 1:25 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using CD25 Rabbit pAb (CAB2984) at a dilution of 1:25 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Flow cytometry: 293F Transfection cells(+) and 293F cells(-) were stained with Rabbit IgG isotype control (AC042, 10 μg/mL, blue line) or CD25 Rabbit pAb (CAB2984, 2.5μg/mL orange line), followed by Goat anti-Rabbit pAb Alexa Fluor 647 (CABS086, 1:600 dilution) staining. Non-fluorescently stained Daudi cells was used as blank control (red line).
