The Integrin alpha 4 (ITGA4/CD49d) Antibody (CAB0696) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high specificity and sensitivity in detecting integrin alpha 4 in human samples, making it suitable for experiments utilizing Western blotting techniques.Integrin alpha 4 plays a pivotal role in various physiological and pathological processes, including immune cell trafficking, tissue repair, and cancer metastasis. By targeting integrin alpha 4, researchers can gain insights into its molecular interactions and functions in different cell types, offering a better understanding of its potential implications in immunology, oncology, and tissue development studies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Integrin alpha 4 (ITGA4/CD49d) Antibody
SKU:
CAB0696
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IA4, CD49D, Integrin alpha 4 (ITGA4/CD49d)
Positive Sample:
Jurkat
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
115kDa
Observed MW:
70kDa/140kDa/150kDa
The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
3676
RRID
AB_2757347
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using Integrin alpha 4 (ITGA4/CD49d) Rabbit pAb (CAB0696) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
