The Integrin beta 3 (ITGB3/CD61) Monoclonal Antibody (CAB19073) is a high-quality antibody developed for reliable detection and analysis of target proteins. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. RRID AB_2862565 Gene ID 3690 Swiss Prot Synonym GT; GT2; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16; BDPLT24; Integrin beta 3 (ITGB3/CD61)
This antibody is validated for use in WB, IP, ELISA, FC (intra) applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Integrin beta 3 (ITGB3/CD61) Monoclonal Antibody
SKU:
CAB19073
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0460
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISAFC (intra)
Recommended Dilution:
WB
1:1000 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
FC
(intra) 1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Junction, Cell Membrane, Cell Projection, Single-Pass Type I Membrane Protein, Focal Adhesion, Lamellipodium Membrane.
Calculated MW:
87kDa
Observed MW:
100kDa
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. RRID AB_2862565 Gene ID 3690 Swiss Prot Synonym GT; GT2; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16; BDPLT24; Integrin beta 3 (ITGB3/CD61)
Purification Method:
Affinity purification
Gene ID:
3690
RRID:
AB_2862565
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Integrin beta 3 (ITGB3/CD61) Rabbit mAb (CAB19073) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunoprecipitation analysis of 300 μg extracts of U-87MG cells using 3 μg Integrin beta 3 (ITGB3/CD61) antibody (CAB19073). Western blot was performed from the immunoprecipitate using Integrin beta 3 (ITGB3/CD61) antibody (CAB19073) at a dilution of 1:1000.
