The IRF5 Monoclonal Antibody (CAB11106) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. RRID AB_2861503 Gene ID 3663 Swiss Prot Synonym SLEB10; IRF5
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
IRF5 Monoclonal Antibody
SKU:
CAB11106
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC0525
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IP
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. RRID AB_2861503 Gene ID 3663 Swiss Prot Synonym SLEB10; IRF5
Purification Method:
Affinity purification
Gene ID:
3663
RRID:
AB_2861503
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using IRF5 Rabbit mAb (CAB11106) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 5min.
Immunoprecipitation analysis of 600 μg extracts of Mouse thymus using 3 μg IRF5 antibody (CAB11106). Western blot was performed from the immunoprecipitate using IRF5 antibody (CAB11106) at a dilution of 1:500.
