The ISG15 Monoclonal Antibody (CAB2416) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for Western blot applications. By binding to the ISG15 protein, this antibody enables precise detection and analysis in a variety of cell types, providing crucial insights for studies in immunology and virology.ISG15, an interferon-stimulated gene, plays a key role in the innate immune response by regulating various cellular processes such as antiviral immunity and inflammation. Its involvement in immune signaling pathways makes it a promising target for research into viral infections, autoimmune diseases, and inflammatory disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ISG15 Monoclonal Antibody
SKU:
CAB2416
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC53794
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
Purification Method
Affinity purification
Gene ID
9636
RRID
AB_2863006
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells using [KD Validated] ISG15 Rabbit mAb (CAB2416) at 1:500 dilution incubated overnight at 4℃. Jurkat cells were treated with IFN-α (100 ng/mL) at 37℃ for 24 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Western blot analysis of lysates from wild type (WT) and ISG15 knockdown (KD) HeLa cells using [KD Validated] ISG15 Rabbit mAb (CAB2416) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
