The ITGB3 Polyclonal Antibody (CAB24844) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody is produced in rabbits and is highly specific for detecting ITGB3 in human samples. It has been validated for use in Western blot applications, allowing for accurate measurement of ITGB3 protein levels in various cell types.Integrin beta-3 plays a crucial role in cell adhesion, migration, and signaling processes, making it a key player in various physiological and pathological conditions.
This antibody is validated for use in WB, FC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ITGB3 Polyclonal Antibody
SKU:
CAB24844
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Junction, Cell Membrane, Cell Projection, Single-Pass Type I Membrane Protein, Focal Adhesion, Lamellipodium Membrane.
Calculated MW:
86kDa/87kDa
Observed MW:
97kDa/130kDa
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
Purification Method
Affinity purification
Gene ID
3690
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ITGB3 Rabbit pAb (CAB24844) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of various lysates using ITGB3 Rabbit pAb (CAB24844) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
