The JAK3 Antibody (CAB0748) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is specifically reactive with human samples, making it ideal for use in Western blot applications. By binding to the JAK3 protein, this antibody enables precise detection and analysis in a variety of cell types, offering insights into the role of JAK3 in immunology and cancer research.JAK3 is a key player in immune system function, particularly in the development and activation of immune cells. Dysregulation of JAK3 signaling has been implicated in various diseases, including cancer, autoimmune disorders, and inflammatory conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
JAK3 Antibody
SKU:
CAB0748
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).
Purification Method
Affinity purification
Gene ID
3718
RRID
AB_2757376
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using JAK3 Rabbit pAb (CAB0748) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Immunofluorescence analysis of TF-1 cells using JAK3 Rabbit pAb (CAB0748) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
