K63-linkage Specific Polyubiquitin Antibody (CAB18164)
The K63-linkage Specific Polyubiquitin Antibody (CAB18164) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically recognizes the K63-linked polyubiquitin chains and is highly reactive with human samples. Validated for use in Western blot applications, this antibody enables the detection and analysis of K63-linked polyubiquitin chains in various cell types.K63-linked polyubiquitin chains play a critical role in diverse cellular processes, including DNA repair, protein degradation, and immune responses.
This antibody is validated for use in ELISA, DB applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
K63-linkage Specific Polyubiquitin Antibody
SKU:
CAB18164
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
ELISADB
Recommended Dilution:
DB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
HeLa, HeLa+MG132, NIH/3T3, C6, Mouse brain, Rat brain
Observed MW:
15-200kDa
Ubiquitination,onetypeofthemostcommonpost-translationalmodification,mediatestheregulationofproteinhomeostasisinvivo.SinceubiquitinitselfcontainsmultiplelysineresiduesandoneN-terminalfreeaminogroup,eighttypesofubiquitinchainscanbeformed.? K63-linked polyubiquitin chains exert nonproteolytic functions in vivo, such as protein trafficking, kinase/phosphatase activation, and DNA damage control, all of which might be important in regulation of cancer survival and development （PMID: 19217402，PMCID: PMC3008764）.
Purification Method
Affinity purification
RRID
AB_2861949
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Dot-blot analysis of all sorts of peptides using K63-linkage Specific Polyubiquitin antibody (CAB18164) at 1:1000 dilution.
