The [KD Validated] BUB1 Monoclonal Antibody (CAB23198) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using hybridoma technology, exhibits high specificity and sensitivity in detecting BUB1 in various experimental settings.BUB1 is a crucial component of the mitotic spindle assembly checkpoint, ensuring accurate chromosome segregation during cell division. Dysregulation of BUB1 has been linked to chromosomal instability and tumorigenesis, making it a promising target for cancer therapy development.With its KD validation, this antibody guarantees reliable and reproducible results in assays such as immunofluorescence, immunoprecipitation, and flow cytometry.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KD Validated] BUB1 Monoclonal Antibody
SKU:
CAB23198
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC59688
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BUB1A, BUB1L, hBUB1, MCPH30, [KD Validated] BUB1
Positive Sample:
HEL, 293T
Cellular Localization:
Chromosome, Nucleus, Centromere, Kinetochore.
Calculated MW:
115KDa/120KDa/122KDa
Observed MW:
130kDa
This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
699
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HEL cells using [KD Validated] BUB1 Rabbit mAb (CAB23198) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
Western blot analysis of lysates from wild type (WT) and BUB1 knockdown (KD) 293T cells using [KD Validated] BUB1 Rabbit mAb (CAB23198) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
