The [KD Validated] IL13 Monoclonal Antibody (CAB23415) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, is specifically designed to target and detect IL13 in various samples, including human tissues and cells. IL13 is known for its role in promoting allergic responses and inflammation, making it a key player in conditions such as asthma and allergy-related diseases. By targeting IL13 with this monoclonal antibody, researchers can accurately measure levels of IL13, analyze its function, and potentially develop therapeutic strategies to modulate its activity.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KD Validated] IL13 Monoclonal Antibody
SKU:
CAB23415
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC61007
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P600, IL-13, [KD Validated] IL13
Positive Sample:
MCF7
Cellular Localization:
Secreted.
Calculated MW:
16kDa
Observed MW:
16kDa
This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
Purification Method
Affinity purification
Gene ID
3596
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type(WT) and IL13 knockdown (KD) MCF7 cells, using [KD Validated] IL13 Rabbit mAb (CAB23415) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
