The KDM3B Antibody (CAB2312) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.KDM3B plays a key role in epigenetic regulation by removing methyl groups from histone proteins, thereby influencing gene expression and chromatin remodeling. Its involvement in processes such as cell proliferation, differentiation, and DNA damage response makes it a relevant target for studies in cancer research and developmental biology.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
KDM3B Antibody
SKU:
CAB2312
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
5qNCA, DIJOS, NET22, C5orf7, JMJD1B, KDM3B
Positive Sample:
HeLa
Cellular Localization:
Nucleus.
Calculated MW:
192kDa
Observed MW:
225kDa
Predicted to enable chromatin DNA binding activity; histone H3-methyl-lysine-9 demethylase activity; and transcription coregulator activity. Predicted to be involved in histone H3-K9 demethylation and regulation of transcription by RNA polymerase II. Located in nucleoplasm. Biomarker of acute lymphoblastic leukemia; breast cancer; colorectal cancer; and lung non-small cell carcinoma.
Purification Method
Affinity purification
Gene ID
51780
RRID
AB_2721243
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Hela cells, using KDM3B Rabbit pAb (CAB2312) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using KDM3B Rabbit pAb (CAB2312) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
