The KDM5A Antibody (CAB7238) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression.
This antibody is validated for use in WB, IP, ChIP, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
KDM5A Antibody
SKU:
CAB7238
Size:
100μL, 20μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPChIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP
5μg antibody for 10μg-15μg of Chromatin
Synonyms:
RBP2, RBBP2, RBBP-2, KDM5A
Positive Sample:
Jurkat, HeLa, MCF7
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
192kDa
Observed MW:
192kDa
This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression.
Purification Method
Affinity purification
Gene ID
5927
RRID
AB_2767786
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using KDM5A Rabbit pAb (CAB7238) at 1:500 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 60s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg KDM5A antibody (CAB7238). Western blot was performed from the immunoprecipitate using KDM5A antibody (CAB7238) at a dilution of 1:500.
