The KIF25 Monoclonal Antibody (PAC063903) is a cutting-edge tool for researchers studying KIF25, a kinesin motor protein involved in intracellular transport and cell division processes. This antibody, derived from mouse hybridoma cells, exhibits high specificity and sensitivity for detecting KIF25 in various experimental settings.Ideal for use in immunofluorescence, immunohistochemistry, and flow cytometry, the KIF25 Monoclonal Antibody allows for precise visualization and quantification of KIF25 expression in different cell types and tissues.
Its versatility makes it an indispensable resource for investigations into cell biology, developmental biology, and cancer research.Given the crucial role of KIF25 in cellular function, understanding its mechanisms of action is essential for unraveling pathways linked to cell division, organelle transport, and disease progression. By using the KIF25 Monoclonal Antibody, researchers can delve deeper into the intricate workings of this protein and its implications for various physiological and pathological processes.
Antibody Name:
KIF25 Antibody (PACO63903)
Antibody SKU:
PACO63903
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Kinesin-like protein KIF25 protein (1-262AA)
IHC image of PACO63903 diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Minus-end microtubule-dependent motor protein (By similarity). Acts as a negative regulator of centrosome separation required to prevent premature centrosome separation during interphase. Required to maintain a centered nucleus to ensure that the spindle is stably oriented at the onset of mitosis. May also act as a negative regulator of amino acid, starvation-induced autophagy.
Synonyms:
Kinesin-like protein KIF25 (Kinesin-like protein 3), KIF25, KNSL3
UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a member of the kinesin-like protein family. Protein family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. However, the particular function of this gene product has not yet been determined. Two alternatively spliced transcript variants which encode products have been described. Other splice variants have been found that lack exon 2 and the initiation codon for translation. [provided by RefSeq, Jul 2008]
