The KIFAP3 Monoclonal Antibody (CAB5216) is a high-quality antibody developed for reliable detection and analysis of target proteins. This high-quality antibody, developed using rabbits, is specifically designed for use in experiments targeting KIFAP3 in human samples.With its superior reactivity and specificity, this antibody is ideal for applications such as Western blotting and immunohistochemistry, enabling accurate detection and analysis of KIFAP3 in various cell types. Its reliability and consistency make it a valuable asset for studies in cell biology, neuroscience, and cancer research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KIFAP3 Monoclonal Antibody
SKU:
CAB5216
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1211
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
22920
RRID
AB_2863489
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using KIFAP3 Rabbit mAb (CAB5216) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunofluorescence analysis of NIH-3T3 cells using KIFAP3 Rabbit mAb (CAB5216) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
