The KLF8 Antibody (CAB16321) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications.KLF8, also known as Kruppel-like factor 8, plays a significant role in cancer development and progression by regulating genes involved in cell cycle control and metastasis. Its dysregulation has been linked to various types of cancer, including breast, lung, and ovarian cancers.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KLF8 Antibody
SKU:
CAB16321
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BKLF3, ZNF741, KLF8
Positive Sample:
U-87MG, HeLa, 293T, Mouse brain, Rat brain
Cellular Localization:
Cytosol, Nucleoplasm.
Calculated MW:
39kDa
Observed MW:
45kDa
This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
11279
RRID
AB_2770108
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using KLF8 Rabbit pAb (CAB16321) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of A431 cells using KLF8 Rabbit pAb (CAB16321) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
