The [KO Validated] ARID2 Antibody (CAB8601) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has been extensively validated for use in various applications, including Western blot, immunofluorescence, and immunoprecipitation.ARID2 is involved in gene regulation and plays a crucial role in controlling cell growth and differentiation. Dysregulation of ARID2 has been linked to various diseases, including cancer, making it a promising target for therapeutic development. The KO-Validated ARID2 Polyclonal Antibody enables researchers to accurately detect and analyze ARID2 expression in different cell types, providing valuable insights into its function and potential therapeutic implications.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KO Validated] ARID2 Antibody
SKU:
CAB8601
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CSS6, p200, BAF200, SMARCF3, ARID2
Positive Sample:
LO2
Cellular Localization:
Nucleus.
Calculated MW:
197kDa
Observed MW:
250kDa
This gene encodes a member of the AT-rich interactive domain (ARID)-containing family of DNA-binding proteins. Members of the ARID family have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and chromatin structure modification. This protein functions as a subunit of the polybromo- and BRG1-associated factor or PBAF (SWI/SNF-B) chromatin remodeling complex which facilitates ligand-dependent transcriptional activation by nuclear receptors. Mutations in this gene are associated with hepatocellular carcinomas. A pseudogene of this gene is found on chromosome1.
Purification Method
Affinity purification
Gene ID
196528
RRID
AB_2863572
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from LO2 cells, using ARID2 Rabbit pAb (CAB8601) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunoprecipitation analysis of 300 μg extracts of 293F cells using 3 μg ARID2 Rabbit pAb (CAB8601). Western blot was performed from the immunoprecipitate using ARID2 Rabbit pAb (CAB8601) at a dilition of 1:1000.
