The [KO Validated] CTCF Monoclonal Antibody (CAB19588) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID AB_2862685 Gene ID 10664 Swiss Prot Synonym MRD21; FAP108; CFAP108; CTCF
This antibody is validated for use in WB, IF/ICC, IP, ChIP, ChIP-seq, ELISA, CUT&Tag applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] CTCF Monoclonal Antibody
SKU:
CAB19588
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0067
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPChIPChIP-seqELISACUT&Tag
Recommended Dilution:
WB
1:1000 - 1:6000
IF
/
ICC
1:2000 - 1:3000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. Ch
IP
5μg antibody for 10μg-15μg of Chromatin Ch
IP
seq 1:50 - 1:100
CUT
&Tag 10⁵ cells /2 μg
Synonyms:
MRD21, FAP108, CFAP108, CTCF
Positive Sample:
Jurkat, HeLa, Mouse lung, 293T, C6
Cellular Localization:
Chromosome, Nucleus, Centromere, Nucleoplasm.
Calculated MW:
83 kDa
Observed MW:
140 kDa
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID AB_2862685 Gene ID 10664 Swiss Prot Synonym MRD21; FAP108; CFAP108; CTCF
Purification Method:
Affinity purification
Gene ID:
10664
RRID:
AB_2862685
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using CTCF Rabbit mAb (CAB19588) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Mouse lung using CTCF Rabbit mAb (CAB19588) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg CTCF antibody (CAB19588). Western blot was performed from the immunoprecipitate using CTCF antibody (CAB19588) at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of 293T cells, using CTCF antibody (CAB19588) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Chromatin immunoprecipitation was performed with 25 μg of cross-linked chromatin from 293T cells using 5 μg of CTCF Rabbit mAb (CAB19588). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of CTCF in the representative genomic region surrounding MYC gene.
Chromatin immunoprecipitation was performed with 25 μg of cross-linked chromatin from 293T cells using 5 μg of CTCF Rabbit mAb (CAB19588). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of CTCF across chromosome 8 (upper panel) and the genomic region encompassing MYC, a representative gene enriched in CTCF (lower panel).
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 2ug μg of CTCF Rabbit mAb (CAB19588), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of CTCF across chromosome 8 (upper panel) and the genomic region encompassing MYC, a representative gene enriched in CTCF (lower panel).
