The [KO Validated] Insulin-degrading enzyme (IDE) Antibody (CAB1630) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is specifically designed for detecting and analyzing IDE in human samples, making it an essential component for studies in diabetes, Alzheimer's disease, and other metabolic disorders.IDE, also known as insulin-degrading enzyme, is a key player in the degradation of insulin and other peptides, contributing to the maintenance of glucose homeostasis and the clearance of amyloid beta, a toxic protein implicated in Alzheimer's disease.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
INSULYSIN, E)
Positive Sample:
DU 145, Mouse liver
Cellular Localization:
Cell Membrane, Cytoplasm, Secreted.
Calculated MW:
118kDa
Observed MW:
118kDa
This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.
Purification Method
Affinity purification
Gene ID
3416
RRID
AB_2861723
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] Insulin-degrading enzyme (IDE) Rabbit pAb (CAB1630) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020).
Western blot analysis of lysates from wild type (WT) and Insulin-degrading enzyme (Insulin-degrading enzyme (IDE)) knockout (KO) HeLa cells, using [KO Validated] Insulin-degrading enzyme (IDE) Rabbit pAb (CAB1630) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of A549 cells using [KO Validated] Insulin-degrading enzyme (IDE) Rabbit pAb (CAB1630). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
