The [KO Validated] IRF3 Monoclonal Antibody (CAB19717) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high specificity and sensitivity for detecting IRF3 in human samples, making it suitable for applications such as Western blotting.IRF3 plays a crucial role in the innate immune response to viral infections, as it regulates the expression of genes involved in antiviral defense mechanisms.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] IRF3 Monoclonal Antibody
SKU:
CAB19717
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC0198
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IIAE7, F3
Positive Sample:
Jurkat, 293T, HeLa
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
47kDa
Observed MW:
57kDa
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The protein plays an important role in the innate immune response against DNA and RNA viruses. Mutations in this gene are associated with Encephalopathy, acute, infection-induced, herpes-specific, 7.
Purification Method
Affinity purification
Gene ID
3661
RRID
AB_2862750
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and IRF3 knockout (KO) HeLa cells, using [KO Validated] IRF3 Rabbit mAb (CAB19717) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Western blot analysis of various lysates using [KO Validated] IRF3 Rabbit mAb (CAB19717) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
