The Cytokeratin 18 (KRT18) Antibody (CAB1022) is a high-quality antibody developed for reliable detection and analysis of target proteins. KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. RRID AB_2757743 Gene ID 3875 Swiss Prot Synonym K18; CK-18; CYK18; Cytokeratin 18 (KRT18)
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cytokeratin 18 (KRT18) Antibody
SKU:
CAB1022
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:5000
IHC-P
1:50 - 1:100
IF
/
ICC
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. RRID AB_2757743 Gene ID 3875 Swiss Prot Synonym K18; CK-18; CYK18; Cytokeratin 18 (KRT18)
Purification Method:
Affinity purification
Gene ID:
3875
RRID:
AB_2757743
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Cytokeratin 18 (KRT18) Rabbit pAb (CAB1022) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.5s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Cytokeratin 18 (KRT18) Rabbit pAb (CAB1022) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of HepG2 cells using Cytokeratin 18 (KRT18) Rabbit pAb (CAB1022) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
