Description
[KO Validated] Laminin beta 1 Antibody (CAB19970)
The [KO Validated] Laminin beta 1 Antibody (CAB19970) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is validated for use in various applications, including Western blotting, immunohistochemistry, and ELISA, and is highly reactive with human samples.LAMB1 is known to be involved in processes such as tissue development, wound healing, and tumor metastasis, making it a target of interest in cancer research and regenerative medicine.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
| Product Name: | [KO Validated] Laminin beta 1 Antibody |
| SKU: | CAB19970 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | EAKL RADE AKQS AEDI LLKT NATK EKMD KSNE ELRN LIKQ IRNF LTQD SADL DSIE AVAN EVLK MEMP STPQ QLQN LTED IRER VESL SQVE VILQ HSAA DIAR AEML LEEA KRAS KSAT DVKV TADM VKEA LEEA EKAQ VAAE KAIK QADE DIQG TQNL LTSI ESET AASE ETLF NASQ RISE LERN VEEL KRKA AQNS GEAE YIEK VVYT VKQS AEDV KKTL DGEL DEKY KKVE NLIA KKTE ESAD ARRK AEML QNEA KTLL AQAN SKLQ LLKD LERK YEDN QRYL EDKA QELA RLEG EVRS LLKD ISQK VAVY STCL | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | CLM, LIS5, 1 |
| Positive Sample: | HeLa |
| Cellular Localization: | Secreted, Basement Membrane, Extracellular Matrix, Extracellular Space. |
| Calculated MW: | 198kDa |
| Observed MW: | 230kDa |
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.
| Purification Method | Affinity purification |
| Gene ID | 3912 |
| RRID | AB_2862878 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from wild type (WT) and Laminin beta 1 knockout (KO) HeLa cells, using [KO Validated] Laminin beta 1 Rabbit pAb (CAB19970) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s. |
