Anti-MTCH2 Antibody (CAB19922)[KO Validated]

SKU:
CAB19922
€129 - €349

Description

Technical Manual

Anti-MTCH2 Antibody [KO Validated]

Product Name:Anti-MTCH2 Antibody [KO Validated]
Product SKU:CAB19922
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a member of the SLC25 family of nuclear-encoded transporters that are localized in the inner mitochondrial membrane. Members of this superfamily are involved in many metabolic pathways and cell functions. Genome-wide association studies in human have identified single-nucleotide polymorphisms in several loci associated with obesity. This gene is one such locus, which is highly expressed in white adipose tissue and adipocytes, and thought to play a regulatory role in adipocyte differentiation and biology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA can give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.

Immunogen:Recombinant protein of human MTCH2.
Sequence:

Email for sequence

Gene ID:23788
Swiss Prot:Q9Y6C9
Synonyms:MTCH2; HSPC032; MIMP; SLC25A50
Calculated MW:33kDa
Observed MW:33KDa
Reactivity:Human, Mouse
Application:WB IHC IF
Recommended Dilution:WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence 
Positive Samples:293T
Cellular Location:Mitochondrion inner membrane, Multi-pass membrane protein

Product Images

Anti-MTCH2 Antibody [KO Validated] (CAB19922)

Western blot analysis of extracts from normal (control) and MTCH2 knockout (KO) 293T cells, using MTCH2 antibody (CAB19922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.

Uniprot Information

UniProt Protein Function:MTCH2: The substrate transported is not yet known. Induces mitochondrial depolarization. Belongs to the mitochondrial carrier family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: membrane; mitochondrion; nucleus

Biological Process: positive regulation of apoptosis

NCBI Summary:This gene encodes a member of the SLC25 family of nuclear-encoded transporters that are localized in the inner mitochondrial membrane. Members of this superfamily are involved in many metabolic pathways and cell functions. Genome-wide association studies in human have identified single-nucleotide polymorphisms in several loci associated with obesity. This gene is one such locus, which is highly expressed in white adipose tissue and adipocytes, and thought to play a regulatory role in adipocyte differentiation and biology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA can give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]
UniProt Code:Q9Y6C9
NCBI GenInfo Identifier:67461088
NCBI Gene ID:23788
NCBI Accession:Q9Y6C9.1
UniProt Secondary Accession:Q9Y6C9,B2R7L8,
UniProt Related Accession:Q9Y6C9
Molecular Weight:33kDa
NCBI Full Name:Mitochondrial carrier homolog 2
NCBI Synonym Full Names:mitochondrial carrier 2
NCBI Official Symbol:MTCH2 
NCBI Official Synonym Symbols:MIMP; HSPC032; SLC25A50 
NCBI Protein Information:mitochondrial carrier homolog 2
UniProt Protein Name:Mitochondrial carrier homolog 2
UniProt Synonym Protein Names:Met-induced mitochondrial protein
Protein Family:Mitochondrial carrier
UniProt Gene Name:MTCH2 
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
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