The KO Validated NANs Polyclonal Antibody (CAB19875) is a valuable tool for researchers studying immune regulation and inflammatory responses. This antibody, raised in rabbits, is specifically designed to target and bind to the NANs protein, allowing for accurate detection and analysis in various cell types. It has been validated for use in Western blot applications, guaranteeing reliable results for your experiments.NANs, also known as a key regulator of immune responses, plays a crucial role in maintaining immune homeostasis and modulating inflammatory pathways.
Research into NANs function is essential for understanding its role in diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. By using the NANs Polyclonal Antibody, researchers can further explore the mechanisms of immune regulation and potentially identify new therapeutic targets for these conditions.Overall, the KO Validated NANs Polyclonal Antibody is a valuable resource for immunology and cancer research, providing accurate and reliable results for studies investigating the role of NANs in immune modulation.
Product Name:
[KO Validated] NANS Rabbit Polyclonal Antibody
SKU:
CAB19875
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-359 of human NANS (NP_061819.2).
This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.
Purification Method:
Affinity purification
Gene ID:
54187
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit pAb (CAB19875) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
