The [KO Validated] RAB27A Antibody (CAB1934) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. RRID AB_2862644 Gene ID 5873 Swiss Prot Synonym GS2; RAM; RAB27; HsT18676; [KD Validated] RAB27A
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] RAB27A Antibody
SKU:
CAB1934
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GS2, RAM, RAB27, HsT18676, [KD Validated] RAB27A
Positive Sample:
U-87MG(KD)
Cellular Localization:
Late Endosome, Lipid-Anchor, Lysosome, Melanosome, Membrane.
Calculated MW:
25kDa
Observed MW:
27kDa
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. RRID AB_2862644 Gene ID 5873 Swiss Prot Synonym GS2; RAM; RAB27; HsT18676; [KD Validated] RAB27A
Purification Method:
Affinity purification
Gene ID:
5873
RRID:
AB_2862644
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type(WT) and RAB27A knockdown (KD) U-87MG cells, using [KD Validated] RAB27A Rabbit pAb (CAB1934) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of HepG2 cells using [KD Validated] RAB27A Rabbit pAb (CAB1934) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
