The [KO Validated] SAMHD1 Antibody (CAB7794) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] SAMHD1 Antibody
SKU:
CAB7794
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DCIP, CHBL2, HDDC1, MOP-5, SBBI88, hSAMHD1, D1
Positive Sample:
HepG2, HeLa, MCF-7, Mouse spleen
Cellular Localization:
Nucleus.
Calculated MW:
72kDa
Observed MW:
72kDa
This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.
Purification Method
Affinity purification
Gene ID
25939
RRID
AB_2863562
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] SAMHD1 Rabbit pAb (CAB7794) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and SAMHD1 knockout (KO) HeLa cells, using [KO Validated] SAMHD1 Rabbit pAb (CAB7794) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg SAMHD1 antibody (CAB7794). Western blot was performed from the immunoprecipitate using SAMHD1 antibody (CAB7794) at a dilution of 1:1000.
