The [KO Validated] SOX2 Monoclonal Antibody (CAB19118) is a high-quality antibody developed for reliable detection and analysis of target proteins. This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). RRID AB_2862611 Gene ID 6657 Swiss Prot Synonym ANOP3; MCOPS3; SOX2
This antibody is validated for use in WB, ELISA, IF-F applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
[KO Validated] SOX2 Monoclonal Antibody
SKU:
CAB19118
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Clone Number:
ARC0449
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-F
Recommended Dilution:
WB
1:1000 - 1:2000
IF-F
1:100 - 1:800
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ANOP3, MCOPS3, SOX2
Positive Sample:
Mouse brain, Rat brain, 293T
Cellular Localization:
Nucleus.
Calculated MW:
34kDa
Observed MW:
35kDa
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). RRID AB_2862611 Gene ID 6657 Swiss Prot Synonym ANOP3; MCOPS3; SOX2
Purification Method:
Affinity purification
Gene ID:
6657
RRID:
AB_2862611
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SOX2 Rabbit mAb (CAB19118) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Confocal imaging of mouse brain(frozen section) using SOX2 Rabbit mAb (CAB19118,at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
