The Cytokeratin 12 (KRT12) Antibody (CAB14746) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically targeting the KRT12 protein, this antibody enables precise detection and analysis in various cell types, making it an essential component for studies in ophthalmology and corneal disease research.KRT12 is a key component of the cytoskeleton in corneal epithelial cells, contributing to the strength and durability of the cornea.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Cytokeratin 12 (KRT12) Antibody
SKU:
CAB14746
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
K12, MECD1, Cytokeratin 12 (KRT12)
Positive Sample:
Mouse eye, Rat eye
Cellular Localization:
Cytoskeleton, Cytosol, Extracellular Exosome.
Calculated MW:
54kDa
Observed MW:
56kDa
KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.
Purification Method
Affinity purification
Gene ID
3859
RRID
AB_2761623
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cytokeratin 12 (KRT12) Rabbit pAb (CAB14746) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
