Description
Lactose Assay Kit
Lactose (C12H22O11), also called milk sugar, is a disaccharide that consists of beta-D-galactose and alpha/beta-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2-8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications.
Assay Genie's lactose assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.
Applications
For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.
Key Features
- Use as little as 20 uL samples. Linear detection range in 96-well plate: 17 to 2000 uM lactose for colorimetric assays and 6 to 100 uM for fluorimetric assays.
Lactose Assay Kit Information
| Kit Includes: | Assay Buffer: 10 mL, Enzyme Mix: Dried Dye Reagent: 120 uL, Lactase: Dried Standard (20 mM Lactose): 1 mL |
| Kit Requires: | Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, optical density plate reader; black 96-well plates and fluorescence plate reader. |
| Method of Detection: | OD570nm, or FL530/585nm |
| Detection Limit: | 6 uM |
| Samples: | Biological (milk), food and agriculture etc |
| Species: | All |
| Protocol Length: | 30 min |
| Size: | 100 tests |
| Storage: | Store all components at -20°C |
| Shelf Life: | 12 months |
