The LEF1 Antibody (CAB0909) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and is suitable for use in Western blot applications. By binding specifically to LEF1, researchers can accurately detect and analyze the protein in various cell types, making it an essential reagent for studies in developmental biology, cancer research, and stem cell biology.
This antibody is validated for use in WB, IP, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LEF1 Antibody
SKU:
CAB0909
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LEF-1, TCF10, TCF7L3, TCF1ALPHA, LEF1
Positive Sample:
BxPC-3, Jurkat, Mouse thymus, Rat testis
Cellular Localization:
Nucleus.
Calculated MW:
44kDa
Observed MW:
25-58kDa
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
51176
RRID
AB_2757454
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LEF1 Rabbit pAb (CAB0909) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded mouse thymus using LEF1 Rabbit pAb (CAB0909) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg LEF1 Rabbit pAb(CAB0909). Western blot was performed from the immunoprecipitate using LEF1 Rabbit pAb(CAB0909) at a dilution of 1:1000.
