The LEFTY1/2 Monoclonal Antibody (CAB2387) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, is optimized for use in Western blot applications and is compatible with human samples. By binding to LEFTY1 and LEFTY2, this antibody enables precise detection and analysis of these important proteins in a variety of cell types.LEFTY1 and LEFTY2 are key factors in the regulation of embryonic development, particularly in the formation of the left-right axis in vertebrates.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LEFTY1/2 Monoclonal Antibody
SKU:
CAB2387
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2149
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LEFTB, LEFTYB, LEFTY1/2
Positive Sample:
HT-29, BxPC-3, Mouse brain, Rat brain
Cellular Localization:
Extracellular Space.
Calculated MW:
34.44kDa
Observed MW:
41kDa
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This gene is closely linked to both a related family member and a related pseudogene. [provided by RefSeq, Aug 2016]
Purification Method
Affinity purification
Gene ID
10637 7044
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LEFTY1/2 Rabbit mAb (CAB2387) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using LEFTY1/2 Rabbit mAb (CAB2387) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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