The Galectin 4/LGALS4 Antibody (CAB17417) is a high-quality antibody developed for reliable detection and analysis of target proteins. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. RRID AB_2770173 Gene ID 3960 Swiss Prot Synonym GAL4; L36LBP; Galectin 4/LGALS4
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Galectin 4/LGALS4 Antibody
SKU:
CAB17417
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
-
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:2000
IF-P
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GAL4, L36LBP, Galectin 4/LGALS4
Positive Sample:
HCT116, mouse large intestine, rat large intestine
Cellular Localization:
Cytosol, Extracellular Space, Plasma Membrane.
Calculated MW:
36kDa
Observed MW:
36kDa
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. RRID AB_2770173 Gene ID 3960 Swiss Prot Synonym GAL4; L36LBP; Galectin 4/LGALS4
Purification Method:
Affinity purification
Gene ID:
3960
RRID:
AB_2770173
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Galectin 4/LGALS4 Rabbit pAb (CAB17417) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of paraffin-embedded human colon carcinoma using Galectin 4/LGALS4 Rabbit pAb (CAB17417) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.