The LIMS1 Antibody (CAB13917) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically targets LIMS1 in human samples and is validated for use in Western blot applications. By binding to LIMS1, this antibody enables precise detection and analysis of the protein in various cell types, making it a key asset for studies in cell biology and cancer research.LIMS1, also known as PINCH-1, plays a crucial role in cell adhesion and migration, particularly in the context of integrin signaling pathways.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
LIMS1 Antibody
SKU:
CAB13917
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
3987
RRID
AB_2760770
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse heart, using LIMS1 Rabbit pAb (CAB13917) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunofluorescence analysis of NIH/3T3 cells using LIMS1 Rabbit pAb (CAB13917) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
