The LRRFIP2 Antibody (CAB17046) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the LRRFIP2 protein, this antibody enables the detection and analysis of LRRFIP2 in various cell types, making it an ideal tool for studies in immunology and inflammatory diseases.LRRFIP2, also known as Leucine-rich repeat flightless-interacting protein 2, is involved in pathways related to immune signaling and cytokine production.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LRRFIP2 Antibody
SKU:
CAB17046
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HUFI-2, LRRFIP2
Positive Sample:
Mouse heart, Mouse skeletal muscle, Rat heart
Calculated MW:
82kDa
Observed MW:
47kDa
The protein encoded by this gene, along with MYD88, binds to the cytosolic tail of toll-like receptor 4 (TLR4), which results in activation of nuclear factor kappa B signaling. The ubiquitin-like protein FAT10 prevents the interaction of the encoded protein and TLR4, thereby inactivating the nuclear factor kappa B signaling pathway. In addition, this protein can downregulate the NLRP3 inflammasome by recruiting the caspase-1 inhibitor Flightless-I to the inflammasome complex.
Purification Method
Affinity purification
Gene ID
9209
RRID
AB_2770217
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using LRRFIP2 Rabbit pAb (CAB17046) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of U-2 OS cells using LRRFIP2 Rabbit pAb (CAB17046) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
