The LSM1 Antibody (CAB12732) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding specifically to the LSM1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in molecular biology and genetic research.LSM1 is an essential component of the mRNA degradation machinery, and its function is critical for the regulation of gene expression.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
LSM1 Antibody
SKU:
CAB12732
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CASM, YJL124C, LSM1
Positive Sample:
Raji, 293T, HeLa
Cellular Localization:
Nucleus.
Calculated MW:
15kDa
Observed MW:
17kDa
This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Increased expression of this gene may play a role in cellular transformation and the progression of several malignancies including lung cancer, mesothelioma and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9.
Purification Method
Affinity purification
Gene ID
27257
RRID
AB_2759575
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using LSM1 Rabbit pAb (CAB12732) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of L929 cells using LSM1 Rabbit pAb (CAB12732) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
