The LTB4 (Leukotriene B4) ELISA Kit is specifically designed for the precise measurement of LTB4 levels in biological samples, such as serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.LTB4 is a potent inflammatory mediator that is involved in various physiological and pathological processes, including inflammation, immune response, and cell migration.
Elevated levels of LTB4 have been implicated in conditions such as asthma, rheumatoid arthritis, and inflammatory bowel disease, highlighting its importance as a biomarker for studying these diseases and developing potential therapeutic interventions.Order the LTB4 (Leukotriene B4) ELISA Kit from AssayGenie today and unlock the key to understanding the role of LTB4 in inflammation and disease pathology.
Product Name:
LTB4 (Leukotriene B4) ELISA Kit
SKU:
UNES00041
Target:
LTB4 (Leukotriene B4) (UNES00041)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LTB4. During the reaction, LTB4 in the sample or standard competes with a fixed amount of LTB4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LTB4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LTB4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
91-105
98-110
Average (%)
103
96
104
1:4
Range (%)
90-103
89-103
94-110
Average (%)
95
94
101
1:8
Range (%)
87-101
88-99
92-106
Average (%)
93
93
99
1:16
Range (%)
84-97
86-97
96-114
Average (%)
89
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.3
119.2
336.5
50.4
116.6
318.6
Standard deviation
3.5
5.0
18.2
2.8
4.8
15.3
C V (%)
6.45
4.19
5.41
5.56
4.12
4.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of LTB4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes LTB4 in samples. No significant cross-reactivity or interference between LTB4 and analogues was observed.
