The LTC4 (Cysteinyl Leukotrienes C4) ELISA Kit is a state-of-the-art assay designed for the precise measurement of LTC4 levels in various biological samples such as serum, plasma, and tissue culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and reliable results for a wide range of research applications. LTC4 is a potent inflammatory mediator involved in allergic and inflammatory responses, playing a critical role in conditions such as asthma, allergic rhinitis, and inflammatory bowel disease.
By detecting LTC4 levels, researchers can gain valuable insights into the pathophysiology of these diseases and potentially identify new therapeutic targets. With its user-friendly protocol and high-quality components, the LTC4 (Cysteinyl Leukotrienes C4) ELISA Kit is an essential tool for studying the role of LTC4 in various disease processes and advancing our understanding of inflammation and immune responses.
Product Name:
LTC4 (Cysteinyl Leukotrienes C4) ELISA Kit
SKU:
AEFI01037
Reactivity:
Universal
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
9.378 pg/mL
Range:
15.625-1000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
89-102%
82-97%
82-94%
EDTA Plasma (n = 10)
85-99%
84-98%
83-98%
Heparin Plasma (n = 10)
85-102%
84-98%
87-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
87-102
96
EDTA Plasma (n = 10)
89-104
95
Heparin Plasma (n = 10)
85-103
95
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Lyophilized)
1 vial
1 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
