The LTD4 (Leukotriene D4) ELISA Kit is designed for the quantitative detection of Leukotriene D4 (LTD4) levels in various biological samples. LTD4 is a potent inflammatory mediator involved in a wide range of cellular activities, including bronchoconstriction, vascular permeability and leukocyte adhesion. It significantly influences the body's immune response by causing smooth muscle contractions and has been implicated in various pathological conditions like asthma, allergic rhinitis, and cardiovascular diseases. Accurate measurement of LTD4 is crucial for understanding its involvement in disease pathogenesis and for the development of targeted therapeutic strategies.
Assay Genie's LTD4 (Leukotriene D4) ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an ideal choice for both research and clinical applications. Trust Assay Genie's LTD4 ELISA Kit for accurate and dependable quantification of this key biomarker in your investigations.
Product Name:
LTD4 (Leukotriene D4) ELISA Kit
SKU:
AEES00003
Target:
LTD4 (Leukotriene D4)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LTD4. During the reaction, LTD4 in the sample or standard competes with a fixed amount of LTD4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LTD4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LTD4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
87-101
93-107
Average (%)
96
92
99
1:4
Range (%)
88-101
89-103
98-112
Average (%)
94
94
104
1:8
Range (%)
92-107
89-103
94-107
Average (%)
97
96
99
1:16
Range (%)
91-102
89-101
97-110
Average (%)
96
94
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
84-99
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.9
9.3
1.0
2.7
10.2
Standard deviation
0.1
0.2
0.3
0.1
0.1
0.4
C V (%)
10
6.9
3.23
10.0
3.7
3.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of LTD4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes LTD4 in samples. No significant cross-reactivity or interference between LTD4 and analogues was observed.
