The LTE4 (Leukotriene E4) ELISA Kit is specifically designed for the sensitive detection of LTE4 levels in biological samples such as serum, plasma, and urine. This kit offers high specificity and accuracy, providing researchers with reliable and consistent results for their studies.Leukotriene E4 is a metabolite produced during the inflammatory response and is known for its role in various inflammatory and respiratory disorders such as asthma and allergic rhinitis.
Monitoring LTE4 levels can help researchers better understand these conditions and potentially develop targeted therapies.Overall, the LTE4 ELISA Kit from Assay Genie is a valuable tool for researchers studying inflammatory pathways, respiratory diseases, and other related fields, providing them with the necessary means to advance their research and make meaningful discoveries.
Product Name:
LTE4 (Leukotriene E4) ELISA Kit
SKU:
UNES00061
Target:
LTE4 (Leukotriene E4) (UNES00061)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LTE4. During the reaction, LTE4 in the sample or standard competes with a fixed amount of LTE4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LTE4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LTE4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-99
99-116
86-100
Average (%)
90
106
93
1:4
Range (%)
90-101
91-107
99-111
Average (%)
96
98
105
1:8
Range (%)
87-102
92-108
97-111
Average (%)
94
99
103
1:16
Range (%)
87-100
85-100
92-106
Average (%)
92
92
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
91
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
92-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.3
209.7
903.1
99.5
225.5
937.6
Standard deviation
5.4
9.4
34.3
6.0
12.2
30.9
C V (%)
5.33
4.48
3.8
6.03
5.41
3.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of LTE4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes LTE4 in samples. No significant cross-reactivity or interference between LTE4 and analogues was observed.
