The Lysozyme (LYZ) Monoclonal Antibody (CAB0641) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. RRID AB_2861471 Gene ID 4069 Swiss Prot Synonym LZM; LYZF1; Lysozyme (LYZ)
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Lysozyme (LYZ) Monoclonal Antibody
SKU:
CAB0641
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC0276
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:1000 - 1:6000
IF-P
1:200 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LZM, LYZF1, Lysozyme (LYZ)
Positive Sample:
Hep G2, RAW 264.7, THP-1, U-937, Mouse lung
Cellular Localization:
Secreted.
Calculated MW:
17kDa
Observed MW:
15kDa
This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. RRID AB_2861471 Gene ID 4069 Swiss Prot Synonym LZM; LYZF1; Lysozyme (LYZ)
Purification Method:
Affinity purification
Gene ID:
4069
RRID:
AB_2861471
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Lysozyme (LYZ) Rabbit mAb (CAB0641) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Confocal imaging of paraffin-embedded Human spleen tissue using Lysozyme (LYZ) Rabbit mAb (CAB0641,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.
