The MANF Polyclonal Antibody (CAB13371) is a valuable tool for researchers studying the role of MANF, a protein involved in cellular protection and maintenance. This antibody, raised in rabbits, is highly specific to human MANF and has been validated for use in various applications, including Western blotting and immunofluorescence.MANF, or mesencephalic astrocyte-derived neurotrophic factor, is known for its neuroprotective and anti-inflammatory properties, making it a key player in neurodegenerative diseases and cellular stress responses.
By targeting MANF with this polyclonal antibody, researchers can analyze its expression and function in different cell types, providing valuable insights into its potential therapeutic applications in neurology and beyond.With its high specificity and reliable performance in experimental settings, the MANF Polyclonal Antibody (CAB13371) is an essential tool for investigating the complex roles of MANF in cellular health and disease, paving the way for new discoveries in the field of molecular biology and biomedicine.
Product Name:
[KO Validated] MANF Rabbit Polyclonal Antibody
SKU:
CAB13371
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-182 of human ARMET/ARP/MANF (NP_006001.4).
The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related.
Purification Method:
Affinity purification
Gene ID:
7873
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts from normal (control) and ARMET/ARP/MANF knockout (KO) 293T cells, using ARMET/ARP/MANF antibody (CAB13371) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30S.
