The MAP1LC3A Monoclonal Antibody (CAB12319) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbits as the host species, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications for the detection of MAP1LC3A protein.MAP1LC3A, a key marker for autophagy, is involved in the formation of autophagosomes and serves as a reliable indicator of autophagic activity within cells. By targeting MAP1LC3A, researchers can gain insights into the mechanisms underlying autophagy and its role in various biological processes, including cellular growth, differentiation, and response to stress.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MAP1LC3A Monoclonal Antibody
SKU:
CAB12319
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2636
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MPSD RPFK QRRS FADR CKEV QQIR DQHP SKIP VIIE RYKG EKQL PVLD KTKF LVPD HVNM SELV KIIR RRLQ LNPT QAFF LLVN QHSM VSVS TPIA DIYE
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3, MAP1LC3A
Positive Sample:
NIH/3T3 treated with Chloroquine, C6 treated with Chloroquine, SH-SY5Y
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.
Purification Method
Affinity purification
Gene ID
84557
RRID
AB_2861653
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3,C6 cells using MAP1LC3A Rabbit mAb (CAB12319) at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated with Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from SH-SY5Y cells using MAP1LC3A Rabbit mAb (CAB12319) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
