The mCherry Rabbit pAb (CABE171) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with mCherry tagged proteins and is validated for use in various experimental techniques such as immunofluorescence, Western blotting, and flow cytometry.mCherry is a versatile tool in cell biology research, allowing for visualization and monitoring of cellular processes in real time. Its bright red fluorescence is easily detectable and compatible with a variety of microscopy systems. The mCherry Rabbit Polyclonal Antibody enables researchers to confidently detect and quantify mCherry expression in different cell types, making it an essential reagent for studies in cell biology, molecular biology, and bioimaging.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
mCherry Rabbit pAb
SKU:
CABE171
Size:
100μL
Reactivity:
Species independent
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
mCherry, mCherry tag, mCherry-tag
Positive Sample:
293F transfected with CT55-mCherry
Observed MW:
55kDa
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CT55-mCherry using mCherry Rabbit pAb (CABE171) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
