The MELK Antibody (CAB10794) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables calcium ion binding activity; non-membrane spanning protein tyrosine kinase activity; and protein serine/threonine kinase activity. Involved in apoptotic process; cell population proliferation; and protein autophosphorylation. Located in cell cortex and plasma membrane. RRID AB_2758228 Gene ID 9833 Swiss Prot Synonym HPK38; MELK
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MELK Antibody
SKU:
CAB10794
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HPK38, MELK
Positive Sample:
293T, SKOV3, HT-29, HeLa, B cells, Mouse thymus, Mouse spleen, Rat testis
Cellular Localization:
Cell Membrane, Peripheral Membrane Protein.
Calculated MW:
75kDa
Observed MW:
75kDa
Enables calcium ion binding activity; non-membrane spanning protein tyrosine kinase activity; and protein serine/threonine kinase activity. Involved in apoptotic process; cell population proliferation; and protein autophosphorylation. Located in cell cortex and plasma membrane. RRID AB_2758228 Gene ID 9833 Swiss Prot Synonym HPK38; MELK
Purification Method:
Affinity purification
Gene ID:
9833
RRID:
AB_2758228
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MELK Rabbit pAb (CAB10794) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
