The Monkey IFABP (Intestinal Fatty Acid Binding Protein) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of IFABP levels in monkey serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it perfect for various research applications.IFABP is a key protein involved in the regulation of fatty acid metabolism and absorption in the intestines.
Dysregulation of IFABP levels has been linked to various gastrointestinal disorders, making it a valuable biomarker for studying and developing potential therapies for these conditions.Overall, the Monkey IFABP ELISA Kit from AssayGenie offers researchers a powerful tool for studying the role of IFABP in monkey physiology and disease, providing valuable insights for future research and drug development efforts.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey IFABP/FABP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey IFABP/FABP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey IFABP/FABP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey IFABP/FABP2. You can calculate the concentration of Monkey IFABP/FABP2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
92-103
97-113
Average (%)
96
97
104
1:4
Range (%)
86-100
91-106
96-107
Average (%)
93
98
101
1:8
Range (%)
90-102
88-102
98-112
Average (%)
95
96
106
1:16
Range (%)
91-104
87-99
95-111
Average (%)
98
94
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
88-98
93
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
0.94
4.57
0.55
0.89
4.34
Standard deviation
0.03
0.04
0.2
0.03
0.04
0.2
C V (%)
6
4.26
4.38
5.45
4.49
4.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey IFABP/FABP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey IFABP/FABP2 in samples. No significant cross-reactivity or interference between Monkey IFABP/FABP2 and analogues was observed.
