Human IFABP (Intestinal Fatty Acid Binding Protein) ELISA Kit
The Human IFABP (Intestinal Fatty Acid Binding Protein) ELISA Kit is a highly sensitive and specific assay designed for the precise detection of IFABP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it suitable for various research applications.IFABP is an important protein involved in the transport of fatty acids in the intestines, playing a critical role in lipid metabolism and absorption. Abnormal levels of IFABP have been implicated in various gastrointestinal disorders, making it a valuable biomarker for studying these conditions and developing potential interventions.Overall, the Human IFABP ELISA kit is a valuable tool for researchers looking to investigate the role of IFABP in health and disease, providing accurate and quantitative measurements for a deeper understanding of intestinal fatty acid binding protein function.
Product Name:
Human IFABP (Intestinal Fatty Acid Binding Protein) ELISA Kit
SKU:
HUES01431
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
