The MonoMethyl-Histone H4-K20 Antibody (CAB2370) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and is optimized for use in immunoprecipitation and ChIP applications. By binding specifically to the monomethylated form of Histone H4 at lysine 20, this antibody enables precise detection and analysis of this histone modification in a variety of cell types.Histone H4 monomethylation at lysine 20 is a key epigenetic mark associated with transcriptional regulation and DNA repair processes. Dysregulation of this modification has been linked to various diseases, including cancer and neurological disorders.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA, DB applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
MonoMethyl-Histone H4-K20 Antibody
SKU:
CAB2370
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBIHC-PIF/ICCELISADB
Recommended Dilution:
WB
1:500 - 1:1000
DB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H4 protein
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
11kDa
Observed MW:
11kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method
Affinity purification
Gene ID
8359
RRID
AB_2764330
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H4-K20 Rabbit pAb (CAB2370) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using MonoMethyl-Histone H4-K20 Rabbit pAb (CAB2370) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human colon using MonoMethyl-Histone H4-K20 Rabbit pAb (CAB2370) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat testis using MonoMethyl-Histone H4-K20 Rabbit pAb (CAB2370) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-K20 Rabbit pAb (CAB2370). Blue: DAPI for nuclear staining.
