The Mouse ACV-A (Activin A) ELISA Kit is specifically designed for the precise measurement of Activin A levels in mouse serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results, which makes it perfect for various research applications.Activin A is a vital member of the TGF-beta superfamily, known for its involvement in various biological processes such as cell differentiation, inflammation, and immune response regulation.
Its dysregulation has been linked to numerous diseases, including cancer, inflammatory disorders, and reproductive issues. Therefore, the Mouse ACV-A ELISA Kit serves as a valuable tool for studying Activin A's role in these conditions and exploring potential therapeutic interventions.
Product Name:
Mouse ACV-A (Activin A) ELISA Kit
SKU:
MOES00622
Target:
Mouse ACV-A (Activin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ACV-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ACV-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ACV-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ACV-A. You can calculate the concentration of Mouse ACV-A in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
94-105
94-108
Average (%)
92
100
100
1:4
Range (%)
91-105
82-95
82-97
Average (%)
99
88
88
1:8
Range (%)
94-108
82-95
87-101
Average (%)
99
89
94
1:16
Range (%)
93-109
81-95
81-96
Average (%)
99
87
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
100
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
269.7
767.83
2199.56
275.09
840.71
2025.95
Standard deviation
16.88
44.46
69.95
14.28
42.88
81.04
C V (%)
6.26
5.79
3.18
5.19
5.1
4.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ACV-A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ACV-A in samples. No significant cross-reactivity or interference between Mouse ACV-A and analogues was observed.
